Patent and Publications

PUBLICATIONS

Swati Singh, Garima Khare, Ritika Kar, Prahlad C. Ghosh and Anil Kumar Tyagi.  Identification of Mycobacterium tuberculosis BioA inhibitors by using structure based virtual screening. Drug Des Devel Ther., 2017, (In Press).

Javeed Ahmad, Aisha Farhana, Rita Pancsa, Simran Arora, Aligari Srinivasan, Anil Kumar Tyagi, Madan Babu, Nasreen Ehtesham, and Seyed Hasnain.  Contrasting function of structured N-terminal and unstructured C-terminal segments of Mycobacterium tuberculosis PPE37 protein. mbio., 2017, (In Press).

Akshay Rohilla, Garima Khare and Anil K. Tyagi. Virtual Screening, pharmacophore development and structure based similarity search to identify inhibitors against IdeR, a transcription factor of Mycobacterium tuberculosis.  Sci Rep., 2017, 7(1):4653.

Ritka Kar, Prachi Nangpal, Shubhita Mathur, Swati Singh and Anil Kumar Tyagi. bioA mutant of Mycobacterium tuberculosis shows severe growth defect and imparts protection against tuberculosis in guinea pigs. PLoS One. , 2017,  12(6):e0179513. doi: 10.1371/journal.pone.0179513.

Garima Khare, Prachi Nangpal and Anil Kumar Tyagi.  Differential roles of two bacterioferritins in maintaining the iron homeostasis in Mycobacterium tuberculosisPLoS One., 2017, 12(1):e0169545. doi: 10.1371/journal.pone.0169545.

Hirawati Deval, Kiran Katoch, Devendra Singh Chauhan, Anil Kumar Tyagi, Rakesh Kumar Gupta, Raj Kamal, Avnish Kumar, Virendra Singh Yadav, Vishwa Mohan Katoch & Tahziba Hussain.  TlyA protein of Mycobacterium leprae: a probable bio-marker of active infection. Lepr Rev (2016) 87, 501– 515

Garima Khare, Priyanka Chauhan, Prachi Nangpal, Ritika Kar and Anil K. Tyagi.  (2016).  Tuberculosis : Current situation, challenges and intervention strategies.  In A. Datta and V.P. Sharma (Eds.), Recent advances in communicable and non-communicable diseases, Capital Publshing Company, New Delhi, India, (pp.77-109).

Deepak Kumar, Khare, G., Beena, Kidwai, S, Tyagi. A.K., Singh, R. & Rawat, D.S. (2015).  Novel isoniazid–amidoether derivatives: synthesis, characterization and antimycobacterial activity evaluation.  Med. Chem. Commun. 6, 131-137.

Syed Asad Rahman, Yadvir Singh, Sakshi Kohli, Javeed Ahmad, Nasreen Z. Ehtesham, Anil K. Tyagi and Seyed E. Hasnain.  Mycobacterium indicus pranii is a distinct genus, not derived from M. intracellulare, and is an organism at an evolutionary transition point between a fast grower and slow grower. mBio 2015., 6(2):e00352-15.DOI:10.1128/Mbio.00352-15.

S. A. Rahman, Singh, Y., Kohli, S., Ahmad, J., Ehtesham, N. Z., Tyagi, A. K., & Hasnain, S. E.  (2014).  Comparative Analysis of Nonpathogenic, Opporltunistic and Totally Pathogenic Mycobacteria Reveal Genomic and Biochemical Variabilities and highlight the Survival Attributes of Mycobacterium tuberculosismBio, 5(6), e02020-14.

Deepal Kumar, Beena, Khare, G., Kidwai, S., Tyagi, A. K., Singh, R., & Rawat, D. S. (2014). Synthesis of novel 1, 2, 3-triazole derivatives of isoniazid and their in vitro and in vivo antimycobacterial activity evaluation.  European Journal of Medicinal Chemistry, 81, 301-313.

Y. Singh, Kohli, S., Sowpati, D. T., Rahman, S. A., Tyagi, A. K., & Hasnain, S.E.  (2014). Gene cooption in Mycobacteria and search for virulence attributes: comparative proteomic analyses of Mycobacterium tuberculosis, Mycobacterium indicus pranii and other mycobacteria.  International Journal of Medical Microbiology. 304(5-6):742-8.

Rupangi Verma Puri, Reddy, P. V., & Tyagi, A.K. (2014).  Apurinic/Apyrimidinic Endonucleases of Mycobacterium tuberculosis Protect against DNA Damage but Are Dispensable for the Growth of the Pathogen in Guinea Pigs.  PloS ONE, 9(5), e92035.

Garima Khare, Praveen Kumar, Anil K Tyagi.  (2013). Whole-Cell Screening-Based Identification of Inhibitors against the Intraphagosomal Survival ofMycobacterium tuberculosis. Antimicrobial Agents and Chemotherapy.  doi:10.1128/AAC.01444-13.

Garima Khare, P. Vineel Reddy, Pragya Sidhwani & Anil K. Tyagi (2013).  KefB inhibits phagosomal acidification but its role is unrelated to M. tuberculosis survival in host.  Scientific Reports.  3 : 3527 | DOI: 10.1038/srep03527.

Priyanka Chauhan, P. Vineel Reddy, Ramandeep Singh, Neetika Jaisinghani, Sheetal Gandotra and Anil K. Tyagi. (2013). Secretory phosphatases deficient mutant of Mycobacterium tuberculosis imparts protection at the primary site of infection in guinea pigs.  PloS ONE. 8(10): e77930. doi:10.1371.

Rupangi Verma Puri, Nisha Singh, Rakesh K. Gupta¤, Anil K. Tyagi.  (2013).  Endonuclease IV Is the Major Apurinic/Apyrimidinic Endonuclease in Mycobacterium tuberculosis and Is Important for Protection against Oxidative Damage.  PLoS ONE 8(8): e71535. doi:10.1371/ journal.pone.0071535.

Rupangi Verma Puri, P. Vineel Reddy, Anil K. Tyagi.  (2013).  Secreted Acid Phosphatase (SapM) of Mycobacterium tuberculosis Is Indispensable for Arresting Phagosomal Maturation and Growth of the Pathogen in Guinea Pig Tissues.  PLoS ONE 8(7): e70514. doi:10.1371/journal.pone.0070514.

P. Vineel Reddy, Rupangi Verma Puri, Priyanka Chauhan, Ritika Kar, Akshay Rohilla, Aparna Khera and Anil K. Tyagi. (2013).  Disruption of mycobactin biosynthesis leads to attenuation of Mycobacterium tuberculosis for growth and virulence.  Journal of Infectious Diseases.  DOI: 10.1093/infdis/jit250.

Priyanka Chauhan, Ruchi Jain, Bappaditya Dey and Anil K. Tyagi. (2013).  Adjunctive immunotherapy with a–crystallin based DNA vaccination reduces tuberculosis chemotherapy period in chronically infected mice.  Scientific Reports.  3: 1821, DOI: 10.1038.

Garima Khare, Prachi Nangpal and Anil K. Tyagi. (2013).  Unique residues at the 3-fold and 4-fold axis of mycobacterial ferritin are involved in oligomer switching.  Biochemistry, 52(10) : 1694-1704.

Ruchi Jain, Bappaditya Dey and Anil K. Tyagi.  (2012).  Development of the first oligonucleotide microarray for global gene expression profiling in guinea pigs: defining the transcription signature of infectious diseases.  BMC Genomics, 13: 520-530.

Vikram Saini, Saurabh Raghuvanshi, Jitendra P. Khurana, Niyaz Ahmed, Seyed E. Hasnain, Akhilesh K. Tyagi and Anil K. Tyagi. (2012).  Massive gene acquisitions in Mycobacterium indicus pranii provide a perspective on mycobacterial evolution.  Nucleic Acids Research.  1-19, doi:10.1093/nar/gks793.

P. Vineel Reddy, Rupangi Verma Puri, Aparna Khera and Anil K. Tyagi.  (2012).  Iron Storage Proteins Are Essential for the Survival and Pathogenesis of Mycobacterium tuberculosis in THP-1 Macrophages and the Guinea Pig Model of Infection.  J. Bacteriol. 194(3):567. DOI: 10.1128/JB.05553-11.

Ruchi Jain, Bappaditya Dey, Aparna Khera, Priyadarshani Srivastava, Umesh D. Gupta, V.M. Katoch, V.D. Ramanathan, Anil K. Tyagi. (2011).  Over-expression of superoxide dismutase obliterates the protective effect of BCG against tuberculosis by modulating innate and adaptive immune responses.  Vaccine.  29: 8118– 8125

Bappaditya Dey, Ruchi Jain, Umesh D. Gupta, V. M. Katoch, V. D. Ramanathan, Anil K. Tyagi. (2011).  A Booster Vaccine Expressing a Latency-Associated Antigen Augments BCG Induced Immunity and Confers Enhanced Protection against Tuberculosis.  PLoS ONE 6(8): e23360.

Garima Khare, Ritika Kar, Anil K. Tyagi. (2011).  Identification of Inhibitors against Mycobacterium tuberculosis Thiamin Phosphate Synthase, an Important Target for the Development of Anti-TB Drugs.  PLoS ONE 6(7): e22441.

Bappaditya Dey, Ruchi Jain, Aparna Khera, Umesh D. Gupta, V.M. Katoch, V.D. Ramanathan and Anil K. Tyagi.  (2011).  Latency antigen a–crystalin based vaccination imparts a robust protection against TB by modulating the dynamics of pulmonary cytokines.  PLoSONE 6(4): e18773. doi:10.1371/journal.pone. 0018773

Garima Khare, Vibha Gupta, Prachi Nangpal, Rakesh K. Gupta, Nicholas K. Sauter and Anil K. Tyagi.  (2011).  Ferritin Structure from Mycobacterium tuberculosis:  Comparative Study with Homologues identifies Extended C-terminus involved in Ferroxidase Activity.  PLoSONE 6(4): e18570. doi:10.1371/journal.pone.0018570.

Purushothaman S, Annamalai K, Tyagi AK, Surolia A (2011) Diversity in Functional Organization of Class I and Class II Biotin Protein Ligase. PLoS ONE 6(3):e16850. doi:10.1371/journal.pone.0016850

Ashish Arora, Nagasuma R. Chandra, Amit Das, Balasubramanian Gopal, Shekhar C. Mande, Balaji Prakash, Ravishankar Ramachandran, Rajan Sankaranarayanan, K. Sekar, Kaza Suguna, Anil K. Tyagi, Mamannamana Vijayan.  (2011).  Structural biology of Mycobacterium tuberculosis proteins: The Indian efforts, Tuberculosis, doi:10.1016/j.tube.2011.03.004

Anil K. Tyagi, Prachi Nangpal, Vijaya Satchidanandam.  (2011).  Development of vaccines against tuberculosis.  Tuberculosis.  Doi:10.1016/j.tube.2011.01.003.

Anuj Kumar Gutpa, Vineel P. Reddy, Mallika Lavania, D.S. Chauhan, K. Venkatesan, V.D. Sharma, A.K. Tyagi and V.M. Katoch.  (2010).  jefA (Rv2459), a drug efflux gene in Mycobacteirum tuberculosis confers resistance to isoniazid and ethambutol.  Indian J. Med. Res. 132: 176-188.

Vibha Gupta, Rakesh K. Gupta, Garima Khare, Dinakar M. Salunke, Avadhesha Surolia and Anil K. Tyagi.  (2010).  Structural ordering of disordered ligand-binding loops of biotin protein ligase into active conformations as a consequence of dehydration.  PloS ONE 5(2): e9222. doi:10.1371/journal.pone.0009222.

Bappaditya Dey, Ruchi Jain, Aparna Khera, Vivek Rao, Neeraj Dhar, Umesh D. Gupta, V.M. Katoch, V.D. Ramanathan and Anil K. Tyagi.  (2010).  Boosting with a DNA vaccine expressing ESAT-6 (DNAE6) obliterates the protection imparted by recombinant BCG (rBCGE6) against aerosol Mycobacterium tuberculosis infection in guinea pigs.  Vaccine.  28: 63-70.

Khare, G., Gupta, V., Gupta, R.K., Gupta, R, Bhat, R. and Anil K. Tyagi.  (2009).  Dissecting the role of critical residues and substrate preferene of a fatty Acyl-CoA synthetase (FadD13) of Mycobacterium tuberculosis.  PLoS ONE 4(12): e8387, doi:10.1371/journal.pone.0008387.

Vibha Gupta, Rakesh K. Gupta, Garima Khare, Dinakar M. Salunke and Anil K. Tyagi.  (2009).  Crystal structure of Bfr A from Mycobacterium tuberculosis: Incorporation of selenomethionine results in cleavage and demetallation of Haem.  PLoS One.  4(11): e8028. doi:10.1371/journal.pone.0008028.

Preeti Sachdeva, Richa Misra, Anil K. Tyagi and Yogendra Singh.  2009.  The sigma factors of Mycobacterium tuberculosis: regulation of the regulators.  FEBS Journal. Doi:10.1111/j.1742-4658.2009.07479.x.

C.M. Santosh Kumar, Garima Khare, C.V. Srikanth, Anil K. Tyagi, Abhijit A. Sardesai and Shekhar C. Mande.  (2009).  Facilitated oligomericzation of mycobacterial GroEL: Evidence for phosphorylation-mediated oligomerization.  J. Bacteriol.  191: 6525-6538.

Vikram Saini, S. Raghuvanshi, G.P. Talwar, N. Ahmed, J.P.  Khurana, S.E. Hasnain, Akhilesh K. Tyagi, and Anil K. Tyagi. (2009). Polyphasic Taxonomic Analysis Establishes Mycobacterium indicus pranii as a Distinct Species. PLoS ONE 4(7): e6263.doc:10.1371/journal.pone. 0006263.

D. Basu, Garima Khare, S. Singh, Anil K. Tyagi, S. Khosla, S.C. Mande.  (2009).  A novel nucleoid-associated protein of Mycobacterium tuberculosis is a sequence homolog of GrotL.  Nucleic Acids Res.  Doi:10.1093/nar/gkp502.

Pooja Arora, Aneesh Goyal, Vivek T. Natarajan, Eerappa Rajakumara, Priyanka Verma, Radhika Gupta, Malikmohamed Yousuf, Omkita A. Trivedi, Debasisa Mohanty, Anil Tyagi, Rajan Sankaranarayanan and Rajesh S. Gokhale.  (2009).  Mechanistic and functional insights into fatty acid activation in Mycobacterium tuberculosis.  Nature Chemical Biology.  5, 166-173.

Ruchi Jain, Bappaditya Dey, Neeraj Dhar, Vivek Rao, Ramandeep Singh, Umesh D. Gupta, V.M. Katoch, V.D. Ramanathan and Anil K. Tyagi.  (2008).  Enhanced and Enduring Protection against Tuberculosis by Recombinant BCG-Ag85C and its Association with Modulation of Cytokine Profile in Lung.  PLoS ONE.  3(12): 3869.doc:10.1371/journal. pone.0003869.

Mohd Akif, Garima Khare, Anil K. Tyagi, Shekhar C. Mande, and Abhijit A. Sardesai (2008). Functional Studies on Multiple Thioredoxins from Mycobacterium tuberculosis. J. Bacteriol. 190: 7087-7095.

Vibha Gupta, Rakesh K. Gupta, Garima Khare, Dinakar M. Salunke and Anil K. Tyagi.  (2008).  Cloning, expression, purification, crystallization and preliminary x-ray crystallographic analysis of bacterioferritin A from Mycobacterium tuberculosis.  Acta Cryst. F 64: 398-401.

Vibha Gupta, Rakesh K. Gupta, Garima Khare, Avadhesha Surolia, Dinakar M. Salunke and Anil K. Tyagi.  (2008).  Crystallization and preliminary x-ray diffraction analhysis of biotin acetyl-CoA carboxylase ligare (BirA) from Mycobacterium tuberculosis.  Acta Cryst.  F 64: 524-527.

A. Farhana, S. Kumar, S.S. Rathore, P.C. Ghosh, N.Z. Ehtesham, Anil K. Tyagi, and S.E. Hasnain.  (2008).  Mechanistic insights into a novel export-import system of Mycobacterium tuberculosis unravel its role in trafficking of iron. PLoS ONE. 3(5): e2087.doi:10.1371/journal.pone. 0002087.

Shruti Jain, Garima Khare, Pushplata Tripathi and Anil K. Tyagi. (2008).  An inducible system for the identification of target genes for a regulator in mycobacteria.  American Journal of Biochemistry and Biotechnology 4(3): 226-230.

Ahmed, N., Saini, V., Raghuvanshi, S., Khurana, J.P., Tyagi, Akhilesh K., Tyagi, Anil K. and Hasnain, S.E.  (2007).  Molecular analysis of a leprosy immunotherapeutic bacillus provides insights into Mycobacterium evolution.  PLoS ONE 2(10): e968. doi:10.1371/journal.pone.0000968.

Azeet Narayan, Preeti Sachdeva, Kirti Sharma, Adesh K. Saini, Anil K. Tyagi and Yogendra Singh.  (2007).  Serine threonine protein kinases of mycobacterial genus: phylogeny to function.  Physiol. Genomics 29: 66-75.

Nisheeth Agarwal and Anil K. Tyagi. (2006).  Mycobacterial Transcriptional Signals: Requirements for Recognition by RNA polymerase and Optimal Transcriptional Activity.  Nucleic Acid Research.  34: 4245-4257.

Ramandeep Singh, Amit Singh and Anil K. Tyagi. (2005).  Deciphering the genes involved in pathogenesis of Mycobacterium tuberculosis. Tuberculosis 85:325-335.

Aparna Khera, Ramandeep Singh, H. Shakila, Vivek Rao, Neeraj Dhar, P.R. Narayanan, C.N. Parmasivan, V.D.Ramanathan and Anil K.Tyagi. (2005).  Elicitation of efficient, protective immune responses by using DNA vaccines against tuberculosis.  Vaccine  23:5655-5665.

Parampal, Deol, Reena Vohra, Adesh K. Saini, Amit Singh, Harish Chandra, Puneet Chopra, Taposh K. Das, Anil K. Tyagi, and Yogendra Singh.  (2005).  Role of Mycobacterium tuberculosis Ser/Thr kinase PknF: implications in glucose transport and cell division.  J. Bacteriol.  187: 3415-3420.

Amit Singh, Radhika Gupta, R. A.Vishwakarma, P.R. Narayanan, C.N. Paramasivan, V. D. Ramanathan and Anil K. Tyagi. (2005).  Requirement of mymA operon for appropriate cell wall ultrastructure and persistence of Mycobacterium tuberculosis in the spleens of guinea pigs.  J. Bacteriol.  187: 4173-4186.

Vivek Rao, Neeraj Dhar, H. Shakila, Ramandeep Singh, Aparna Khera, M. Naseema, C. N. Paramasivan, P. R. Narayananan, V. D. Ramanthan and Anil K. Tyagi. (2005).  Over-expression of the 19kDa lipoprotein of Mycobacterium tuberculosis obliterates the protective efficacy of BCG by polarizing the host immune responses to the Th2 phenotype.  Scand. J. Immunol.  61: 410-417.

V.K. Chaudhary, Kulshresta Abhishek, Gupta Ghata, Verma Nitin, Sampati Kumari, S.K. Sharma, Gupta Amita, A.K. Tyagi. (2005).  Expression and purification of 38-kDa and Mtb81 antigens of Mycobacterium tuberculosis for application in serodiagnosis.  Protein Expr. Purif.  40: 169-176.

Saini, A.K., Maithal, K., Chand, P., Chowdhury, S., Vohra, R., Goyal, A., Dubey, G.P., Chopra, P., Chandra, R., Tyagi, A.K., Singh, Y., Tandon, V.  (2004).  Nuclear localization and in situ DNA damage by Mycobacterium tuberculosis nucleosidediphosphate kinase.  J. Biol. Chem. 279: 50142-50149.

Chopra, P., Koduri, H., Singh, R., Koul, A., Ghildiyal, M., Sharma, K., Tyagi, A.K., Singh, Y.  (2004).  Nucleoside diphosphate kinase of Mycobacterium tuberculosis acts as GTPase-activating protein for Rho-GTPases.  FEBS Lett. 571: 212-216.

Neeraj Dhar, Vivek Rao and Anil K. Tyagi.  (2004).  Immunogenicity of recombinant BCG vaccine strains overexpressing components of the antigen 85 Complex of M. tuberculosis.  Med Microbiol Immunol.  193: 19-25.

Ramandeep Singh, Vivek Rao, H. Shakila, Radhika Gupta, Aparna Khera, Neeraj Dhar, Amit Singh, Anil Koul, Yogendra Singh, M. Naseema, P.R. Narayanan, C.N. Paramasivan, V.D. Ramanathan and Anil K. Tyagi.  (2003). Disruption of mptpB impairs the ability of Mycobacterium tuberculosis to survive in guinea pigs.  Molecular Microbiology 50(3): 751-762.

Chopra P, Singh B, Singh R, Vohra, R, Koul A, Meena LS, Koduri H, Ghildiyal M, Deol P, Das TK, Tyagi AK, Singh Y.  (2003). Phosphoprotein phosphatase of Mycobacterium tuberculosis dephosphorylates serine-threonine kinases PknA and PknB.  Biochem Biophys Res Commun.  311(1): 112-120.

Vivek Rao, Neeraj Dhar and Anil K. Tyagi.  (2003). Modulation of host immune responses by over-expression of immunodominant antigens of M. tuberculosis in BCG.  Scand J Immunol.  58(4): 449-461.

Amit Singh, Shruti Jain, Seema Gupta, Taposh Das and Anil K. Tyagi. (2003).  mymA operon of Mycobacterium tuberculosis : its regulation and importance in the cell envelope.  FEMS Microbiol. Lett. 227(1): 53-63.

Nisheeth Agarwal and Anil K. Tyagi. (2003). Role of 5`TGN3` Motif in the Interaction of Mycobacterial RNA Polymerase with a Promoter of “Extended –10” class.  FEMS Microbiol. Lett. 225: 75-83.

Neeraj Dhar, Vivek Rao and Anil K. Tyagi. (2003).  Skewing of the Th1/Th2 responses in mice due to variation in the level of expression of an antigen in a recombinant BCG system.  Immunol Lett.  88(3): 175-184.

Puneet Chopra, Anubha Singh, Anil Koul, S. Ramachandran, Karl Drilica, Anil K. Tyagi and Yogendra Singh.  (2003).  Cytotoxic activity of nucleoside diphosphate kinase secreted from Mycobacterium tuberculosis.  Eur. J. Biochem.  270, 625-634.

Koul, A., Choidas, A., Tyagi, A.K., Drilca, K., Singh, Y. and Ullrich, A.  2001.  Serine/threonine protein kinases PknF and PknG of Mycobacterium tuberculosis: Characterization and Localization.  Microbiology (U.K.).  147, 2307-2314.

Dhar Neeraj, Rao, Vivek and Tyagi, A.K. 2000.  Recombinant BCG approach for development of vaccines: cloning and expression of immunodominant antigens of M.tuberculosis.  FEMS Microbiol. Lett., 190(2), 309-316.

Koul, Anil, Choidas, Axel, Treder, Martin, Tyagi, A.K., Drlica, Karl, Singh, Y. and Ullrich, Axel.  2000.  Cloning and characterization of secretory tyrosine phosphatases of Mycobacterium tuberculosis.  J. Bacteriol. 182(19), 5425-5432.

Gupta, S., Jain, S. and Tyagi, A.K. 1999.  Analysis, expression and prevalence of the M.tuberculosis homolog of bacterial virulence regulating proteins. FEMS. Microbiol. Lett. 172, 137-143.

Bashyam, M.D., and Tyagi, A.K. 1998.  Identification and analysis of "Extended-10" promoters from Mycobacteria.  J. Bacteriol. 180, 2568-2573.

DasGupta, S.K., Jain, S., Kaushal, D., and Tyagi, A.K. 1998.   Expression systems for study of mycobacterial gene regulation and development of recombinant BCG vaccines.  Biochem. Biophys. Res. Commun. 246, 797-804.

Jain, S., Kaushal, D., Dasgupta, S.K., and Tyagi, A.K. 1997.   Construction of shuttle vectors for genetic manipulation and molecular analysis of mycobacteria.  Gene 190, 37-44.

Bashyam, M.D., Kaushal, D., Dasgupta, S.K., and Tyagi, A.K. 1996.   A study of the Mycobacterial transcriptional apparatus: Identification of novel features in promoter elements.  J. Bacteriol. 178, 4847-4853.

Sarkar, N.K., Shankar, S., and Tyagi, A.K. 1995.  Polyamines exert regulatory control on mycobacterial transcription - A study using RNA polymerase form Mycobacterium phlei.  Biochemistry and Molecular Biology International 35, 1189-1198.

Bashyam, M.D. and Tyagi, A.K. 1994.  An efficient and high-yielding method for isolation of RNA from mycobacteria.  Biotechniques 17 : 834-836.

DasGupta, S.K., Bashyam, M.D., and Tyagi, A.K. 1993. Cloning and assessment of mycobacterial promoters by using a plasmid shuttle vector.  J. Bacteriol. 175, 5186-5192.

Gupta, S., and Tyagi, A.K. 1993. Sequence of a newly identified Mycobacterium tuberculosis gene encoding a protein with sequence homology to virulence regulating proteins.  Gene 126, 157-158.

Shankar, S., and Tyagi, A.K. 1993. Purification and Characterization of restriction endonuclease MgoI from Mycobacterium gordonae, Gene 131, 153-154.

Shankar, S., and Tyagi, A.K. 1993.  MchAI and MchAII, two class-II restriction endonucleases from Mycobacterium chelonei.  Gene 132, 119-123.

Shankar, S., and Tyagi, A.K. 1992.  MhaAI, a novel isoschizomer of PstI from Mycobacterium habana recognizing 5'-CTGCA/G-3'.  Nucleic Acids Res. 20, 2891.

Shankar, S.,and Tyagi, A.K. 1992.  MfoAI a novel isoschizomer of HaeIII from Mycobacterium fortuitum recognizing 5'-GG/CC-3'.  Nucleic Acids Res. 20, 2890.

Tyagi,  J.S.,  Tyagi,  A.K. and Bhargava, S. 1990.   Transfer RNA genes in mycobacteria :  organization and molecular cloning.  Trop Med. Parasitol 41, 294-296.

Bhargava, S., Tyagi, A.K. and Tyagi, J.S.  1990.  tRNA genes in mycobacteria: organization and molecular cloning.  J. Bacteriol. 172, 2930-2934.

Balasundaram,  D.,  and Tyagi,  A.K., 1989.  Modulation of Arginine decarboxylase  activity from Mycobacterium smegmatis :  Evidence for pyridoxal  -5'- phosphate  mediated  conformational changes in the enzyme.  Eur. J. Biochem. 183, 339-345.

Balasundaram,  D., and Tyagi, A.K. 1988. Regulation of ornithine decarboxylase  from Mycobacterium smegmatis Arch. Biochem. Biophys. 264, 288-294.

Jain,  A., and Tyagi, A.K. 1987.  Role of polyamines in the synthesis of RNA in mycobacteria.  Mol. Cell. Biochem. 78, 3-8.

Bhutani,  V.,  Kumar,  V.,  Tyagi,  A.K. and Misra,  U.K. 1987. Changes  of fatty acid composition in pancreas of  rats fed inadequate dietary protein.  Nutrition Research 7, 763-770.

Tyagi,  A.K.,  Wickner, R.B., Tabor, C.W. and Tabor, H.  1984.  Specificity  of polyamine requirements for the replication and maintenance of different double-stranded RNA plasmids in Saccharomyces cerevisiae.   Proc.  Natl. Acad. Sci. USA. 81, 1149-1153.

Tyagi, A.K., Tabor,  H.  and Tabor, C.W. 1982.  Inactivation of yeast ornithine decarboxylase by polyamines in vivo does not result  from  the incorporation of  polyamines  into enzyme protein.  Biochem. Biophys. Res. Commun. 109, 533-540.

Tyagi,  A.K.,   Tabor,  C.W.  and  Tabor,  H.  1981.  Ornithine decarboxylase  from Saccharomyces cerevisiae : Purification,  properties and regulation of activity.  J. Biol. Chem. 256, 12156-12163.

Tyagi,  A.K.,  Thake,  D.C.,  Mcgee,  E.  and Cooney, D.A. 1981.  Determinants of  the  toxicity of  L-alanosine  to various organs of the mouse.  Toxicology 21, 59-69.

Tyagi,  J.S., Tyagi, A.K. and Venkitasubramanian, T.A.  1981.  Some factors regulating [1-14C] acetate incorporation into aflatoxins by spheroplast and spheroplasts lysates from Aspergillus parasiticus.  Toxicon 19, 445-454.

Tyagi,  J.S.,  Tyagi,  A.K. and Venkitasubramanian,  T.A. 1981.  Preparation and properties of spheroplasts from Aspergillus parasiticus with special reference to the denovo synthesis of Aflatoxins. J. Appl. Bacteriol. 50, 481-491.

Tyagi,  A.K.,  Cooney,  D.A., Jayaram, H.N., Swiniarski, N.K. and  Johnson  R.K.  1981.   Studies on the mechanism of resistance  of selected murine tumors to L-alanosine.   Biochem. Pharmacol. 30, 915-924.

Tyagi,  A.K. and Cooney, D.A.  1980.  Identification of the antimetabolite of L-Alanosine, L-alanosyl-5-Amino-4-imidazolecarboxylic acid ribonucleotide in tumors and assessment of its inhibition of adenylosuccinate synthetase.  Cancer Res. 40, 4390-4397.

Kensler,  T.W.,  Erlichman,  C., Jayaram, H.N., Tyagi, A.K., Ardalan,  B.  and Cooney,  D.A.  1980.  Peripheral leukocytes as indicators of the enzymatic effects of N-(phosphonoacetyl)-L-aspartic  acid (PALA)  on  human  L-aspartate transcarbamoylase (ATCase) activity.  Cancer Treatment Reports 64, 967-973.

Park, K.W.,  Tyagi A.K.,  and Cooney,  D.A.  1980. A radiometric technique for the measurement of adenylosuccinate  lyase.  J. Biochem. Biophys. Methods 2, 291-297.

Anandaraj, S.A., Jayaram, H.N., Cooney, D.A.; Tyagi, A.K. Han, N., Thomas, J.H., Chitnis, M., and Montgomery, J.A. 1980.  Interaction of L-alanosine (NSC-153353) with enzymes metabolizing L-aspartic  acid, L-glutamic acid and their amides. Biochem. Pharmacol. 29, 227-245.

Tyagi,  A.K.,  Cooney, D.A., Bledsoe, M. and Jayaram, H.N.  1980. Two complementary radiometric methods for the measurement of 5-amino-4-imidazole-N-succino-carboxamide ribonucleotide synthetase (SAICAR synthetase). J. Biochem. Biophys. Methods 2, 123-132.

Jayaram, H.N., Tyagi, A.K., Anandaraj, S., Montgomery, J.A., Kelley, J.A., Kelley, J., Adamson, R.H. and Cooney, D.A.  1979. Metabolites of alanosine, an antitumor antibiotic.  Biochem. Pharmacol. 28, 3551-3566.

Tyagi, A.K., Jayaram, H.N., Anandaraj, S., Taylor, B. and Cooney D.A. 1979.  Use of Automated chromatography on the amino acid analyzer with lithium citrate buffers to separate nucleic acid bases, nucleosides, nucleotides and their precursors.  J. Biochem. Biophys. Methods. 1, 221-226.

Nizamuddin, A., Tyagi, A.K., Padh, H. and Venkitasubramanian, T.A. 1978.  Alkaline phosphatase activity in Mycobacterium smegmatis.  Ind. J. Chest. Dis. 20, 11-20.

Tyagi, A.K., Siddiqui, F.A. and Venkitasubramanian, T.A.  1977.  Studies on the purification and characterization of malate dehydrogenase from Mycobacterium phlei.   Biochem. Biophys. Acta 485, 255-267.

Tyagi, A.K. 1976.  Sources of energy production in Mycobacterium tuberculosis.  Ind. J. Chest Dis. 18, 250-255.

Tyagi, A.K., Reddy, T.L.P. and Venkitasubramanian, T.A. 1976.  Effect of oxygen tension on oxidative phosphorylation in Mycobacterium phlei.  Ind. J. Biochem. Biophys. 13, 93-95.

Tyagi, A.K., Reddy, T.L.P. and Venkitasubramanian, T.A.  1976. Oxidative phosphorylation in Mycobacterium tuberculosis BCG. Ind. J. Biochem. Biophys. 13, 43-45.

Tyagi, A.K. Reddy, T.L.P. and Venkitasubramanian, T.A.  1976.  Site of action of nonheme iron in the malate (flavine adenine dinucleotide) pathway of Mycobacterium phlei. Can. J. Microb. 22, 1054-1057.

Books chapters and reviews

Anil K. Tyagi, Bappaditya Dey and Ruchi Jain (2009).  Tuberculosis vaccine development: Current status and future expectations.  In: Sharma, S.K., Mohan, A. (eds.). Tuberculosis, 2nd ed., New Delhi: Jaypee Brothers, Medical Publishers pg 918-946.

Ruchi Jain, Bappaditya Dey and Anil K. Tyagi.  (2008).  Role of vaccines and immuno-modulation in tuberculosis.  In: O.P. Sood and S.K. Sharma (eds.), Round Table Conference Series, Challenges of MDR/ XDR Tuberculosis in India, Ranbaxy Science Foundation, New Delhi, pg.93-102.

Anil K. Tyagi, Ramandeep Singh and Vibha Gupta.  (2008).  Role of Mycobacterial kinases and Phosphatases in Growth and Pathogenesis, in Reyrat, J.M. and Daffe, M. (Eds.): The Mycobacterial Cell Envelope, ASM Press, Washington DC, USA, pp.323-343.

Ruchi Jain and Anil K. Tyagi.  (2005).  New technology forces microbes to reveal their secrets.  In: T. Satyanarayana and B.N. Johri (eds.).  Microbial Diversity: Current Perspectives and Potential Applications, I.K. International Pvt. Ltd., India, 1053-1074.

Anil K. Tyagi, Bappaditya Dey and Ruchi Jain.  (2005).  Development of vaccine against tuberculosis.  In: N.K. Mehra and S.K. Sharma and O.P. Sood (eds.) “HIV and Tuberculosis : Co-infection” under “Round Table Conference Series”, Ranbaxy Science Foundation, India, 15: 149-153.

Ruchi Jain, Neeraj Dhar, Vivek Rao and Anil K. Tyagi. (2004).  Development of candidate vaccines for protection against tuberculosis.  In: Pawan Sharma (eds.).  Tuberculosis:  Current Research Trends, Allied Publishers Pvt. Ltd., India,  138-158.

Anil K. Tyagi and Aparna Khera.  (2004).  Protection against tuberculosis:  How close are we to a perfect vaccine?  Current Science.  86: 154-166.

Anil K. Tyagi and Neeraj Dhar.  (2003).  Recent Advances in Tuberculosis Research in India.  In: T.K. Ghose and P. Ghosh (eds.): “Biotechnology in India” under the series “Advances in Biochemical Engineering/Biotechnology”, Springer Verlag 84, 211-273.

Anil K. Tyagi (2000).  A Perfect Vaccine Against Tuberculosis: Premises and Promises.  In: Sushma Gupta and O.P. Sood (eds.) “Tuberculosis” under “Round Table Conference Series”, Ranbaxy Science Foundation, India, 7: 135-139.

Tyagi, A.K., Das Gupta, S.K. and Jain, S.  2000. Gene Expression : Reporter Technologies.  In: Graham F. Hatfull and William R. Jacobs Jr. (eds.) : Molecular Genetics of Mycobacteria, ASM Press, pp.131-147.

Tyagi, A.K. and Jain, S.   1999.  Mycobacterium tuberculosis : Studies on Gene Regulation, Pathogenesis and Development of Recombinant BCG Vaccines.  In : R.C. Mahajan and Amu Therwath (eds.), Multidrug Resistance in Emerging and Re-emerging diseases.  Narosa Press, pp.109-124.

Tyagi, A.K. 1998.  Mycobacterium tuberculosis : Studies on gene regulation, pathogenesis and development of recombinant BCG vaccines.  In: Tuberculosis Research: Into the 21st Century, pp.29-42.

Tyagi, A.K. and Cooney,  D.A.   1984.  Biochemical pharmacology, metabolism, and mechanism of action of L-alanosine, a novel natural antitumor agent, in Caratini, S., Goldin, A., Howking, F., and Kopin, I.J.  (Eds.)  :  Advances in Pharmacology and Chemotherapy Vol.  20, Academic Press, New York, pp.69-121.

Tabor, C.W., Tabor, H.  and Tyagi,  A.K. 1983.  Biochemical and genetic studies of polyamines in Saccharomyces cerevisiae. In Bachrach, U., Kaye. A. and Chayen, R. (Eds.) : Advances in Polyamine Research Vol.  4, Raven Press, New York, pp. 467-478.

Tyagi A.K., Tabor, C.W.   and  Tabor,  H.  1983.  Ornithine decarboxylase (Saccharomyces cerevisiae), Tabor, H. and Tabor C.W. (eds.). Methods in Enzymology, Vol. 94, Academic Press, New York. pp. 135-139.

Tyagi, A.K. and Cooney,  D.A.  1983.  Prospects for the chemotherapy of cancer using analogs of L-aspartic acid.  Trends Pharmacol Sci., 4, 299-304.

Tabor, C.W., Tabor, H., Tyagi, A.K. and Cohn, M.S.  1982.  The biochemistry, genetics, and   regulation of polyamine biosynthesis in Saccharomyces cerevisiae.   Fed.  Proc. 41 (review article), pp.3084-3088.

Balasundaram, D. and Tyagi, A.K. 1991.  Polyamine - DNA Nexus : Structural Ramifications and Biological Implications. Mol. Cell. Biochem. 100, 129-140.

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